rp1954 wrote:This paper's results had G12V as the marker of the most successfully treated line (SW480) )of the mutant CRC cell lines tested (vs LoVo for KRAS G13D and HT29 for BRAF V600E), for mutant CRC cells treated with vitamin C.
IV vitamin C temporarily reaches much higher levels in the arteries and veins but (dehyro)ascorbate levels will be lower perfused and transported further in.
Our viable cell lab's sensitivity tests showed that multiple adjuncts (5FU chemo + 2 natural) were necessary to kill less sensitive cancer cells. It seemed surprising then that the resistant CRC cells were not very -iri or -oxi sensitive even without prior treatment. Tissue labs struggled with mutant cell identification, which I suspect has to do with all that cimetidine and IV vitamin C distorting results with those cells that didn't disintegrate and also yielded less CA19-9. However higher bloodlevels of CA199 are often associated with KRAS/BRAF mutations, where serum CA199 is easier to monitor frequently anyway.
In any case, parallel to your situation before surgery, we used oral chemo (5FU base), IV vitamin C, cimetidine and heavy duty supplements in the run up to surgery. Although we used higher vitamin dosages, this list looks useful (a different list than my usual LEF links). Multiple chemo and supplement ajuncts substantially drove down the most common Kras indicator, CA199, before surgery. In 1-2 months, a working dosage eliminated 85%-95% of the CA19-9 blood level associated with the conglomerated mass that was finally removed. Since then, we also found celebrex very useful to improve oral immunochemo results.
zephyr wrote:I don't know if this will help. It won't directly answer your question but it might approach it from another angle. I just learned that my tumor (removed over a year ago) had a KRAS 13 mutation. I've been on chemo for over a year and I was in a pretty dark place a while back; the chemo - everything, really - one day it was just all too much. I followed up on a recommendation from another CRC patient to see a naturopathic doc who specialized in oncology. Her goal was to build my immune system and help me manage the chemo side effects; my oncologist was familiar with her practice and fully supportive. She started giving me vitamin C by IV and I started feeling better almost immediately. The next chemo round was noticeably easier, the one after that even better, and I began to feel that she had given me back my life. I can tell my immune system is back in the game; I'm feeling better, I'm healing faster, I'm sleeping better, and I've pulled out of a depression that was beginning to scare me. When I handed her a copy of the genetic testing report earlier this week, she commented that vitamin C targets KRAS. I also noticed on the printed recommendations she gave me after our first visit that IV vitamin C is synergistic with 5FU. As a disclaimer, I'm also on a handful of other supplements, as well as mistletoe injections. I believe all are contributing to my overall mental and physical health, but also that vitamin C was the game changer. Other cancer patients I've met, my IV-chair buddies, have expressed similar feelings. The only side effect we've noticed is that sometimes you feel a bit high after an infusion, not goofy-high just happy and energetic. Maybe euphoric is a better word.
How is all this affecting what remains of my cancer? Don't know but I have another scan in 2 weeks. It might be too soon to notice much difference but ... fingers crossed. Even if I don't show improvement this time around, the change in my quality of life is worth it.
I'm not trying to give anyone medical advice, just relating my own experience is case it's beneficial to someone else. Your mileage may vary.
NHMike wrote:I looked through the KRAS 13 mutations and didn't see anything bad like BRAF V600E and G12V but do you know your mutation? There are six listed on the MGH Targeted Cancer page and I didn't see major issues with them.
SweetC80 wrote:This is really interesting. I never thought to look into my mom's specific type of mutation. I've looked back at her pathology and see it is G12C, so I'm off to research.
NHMike wrote:SweetC80 wrote:This is really interesting. I never thought to look into my mom's specific type of mutation. I've looked back at her pathology and see it is G12C, so I'm off to research.
I just tried a Twitter search for KRAS G12C and there has been developmental work on this mutation this past year. The research seems interesting but all of the articles about it are behind paywalls.
Here's an example:
http://www.sciencedirect.com/science/ar ... 5617302313
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